Methods of screening and production of new antigen binding molecules for and its application to agricultural fields treatment of new antigen-binding molecules, diagnosis, prevention, enzymes, as well as industry

摘要

The present invention, antigen binding, such as a fragment the antibody, including the class antibody double-stranded and single, major histocompatibility complex (MHC) molecules, T cell receptors (TCR), or an antibody domain, I discloses a method of generating a library or set of nucleic acid encoding a site. By changing the template nucleic acid saturation mutagenesis, synthetic ligation reassembly, such as by a combination thereof, (for example, Fc domain or Fab) invention, as fragments of antibody or (specific domain) domain specific antibodies and provides methods of generating a variant antigen binding sites are. Stability, sustainability, expressing,-forming enzyme activity of, affinity, binding force, localization, and the present invention at a certain plane, immunological way or the other, to produce a humanized antibody or whole human there is provided a method for evolving these antibodies in order to obtain optimal properties regarding qualities. The polypeptides produced by these methods, are analyzed using a capillary array platform of the new, the platform provides a very high throughput screening unprecedented.