Process for the preparation of polynucleotide library using the linker and oligonucleotide linker containing a variable projection site

摘要

(In the formula, and a derivative thereof, or U, A, C, G, or T, N is an integer of 1 or more n) and formula oligonucleotides constant region (N) n oligo represented by linker group or linker comprising a nucleotide variable sites. Polynucleotide group - or linker polynucleotide - linker including a first target polynucleotide chain bound to the linker and linker group or the linker. Method for preparing polynucleotides - linker with linker group or the linker, and methods of preparing the linker or the linker group. cDNA library preparation method using the linkers and linkers, alternative available G- tailing the cDNA library preparation method is provided.