Synthetic peptides useful in biological assays for detection of infection by group O HIV-1 virus

摘要

Synthetic peptides of the monomer type with 13 to 33 amino acids, in linear form or in a form cyclized by means of inter-cysteine disulphide bridges, have the general formula (I): ?in-line-formulae description="In-line Formulae" end="lead"?Delta-Z-TrpGlyCys-Theta-CysTyrThrSer-Omega (I)?in-line-formulae description="In-line Formulae" end="tail"? wherein Delta is a biotinyl radical, a biocytinyl radical, a hydrogen atom, an acetyl (CHSUB3/SUBCO-) radical, an aliphatic chain which may contain one or two thiol, an aldehyde functional group, or an amine functional group, Z represents peptide sequence -XiSUB1/SUB-Ser-XiSUB2/SUB-, -XiSUB1/SUB-Gln-XiSUB2/SUB-, or -XiSUB1/SUB-Asn-XiSUB2/SUB-, wherein -XiSUB1 /SUBrepresents a peptide sequence of 0 to 9 amino acids and -XiSUB2 /SUBrepresents a peptide sequence of 0 to 5 amino acids, Theta is -Arg Gly Arg Leu Ile-(SEQ ID NO: 15), -Arg Gly Arg Leu Val-(SEQ ID NO: 16), -Arg Gly Lys Leu Ile- (SEQ ID NO: 17), -Arg Gly Lys Leu Val-(SEQ ID NO: 18), -Lys Gly Arg Leu Ile-(SEQ ID NO: 19), or -Lys Gly Arg Leu Val-(SEQ ID NO: 20), Omega, attached to the -CO- group of serine, is a hydroxyl (-OH) radical, an amino (-NHSUB2/SUB) radical, an alkoxy radical having 1 to 6 carbon atoms, a peptide sequence of formula Val-Sigma-Psi wherein Sigma represents a sequence of formula -(AASUB1/SUB)-Trp Asn-(AASUB2/SUB)-(AASUB3/SUB) wherein (AASUB1/SUB) represents an amino acid different from lysine, (AASUB2/SUB) represents an amino acid, and (AASUB3/SUB) is serine or a threonine residue, and Psi, attached to the -CO- residue of the free AASUB3 /SUBamino acid, is OH, NHSUB2/SUB, or an alkoxy radical having from 1 to 6 carbon atoms, and a peptide sequence of formula -Val-Psi wherein Psi, attached to the -CO- residue of valine, is OH, NHSUB2/SUB, or an alkoxy radical having from 1 to 6 carbon atoms.