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Vaccinia topoisomerases I-based assays for detection of specific DNA breaks
Vaccinia topoisomerases I-based assays for detection of specific DNA breaks
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机译:基于牛痘拓扑异构酶I的检测特定DNA断裂的方法
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摘要
Several types of DNA cuts are used as markers of apoptosis for detection of apoptotic cells in situ. The present invention includes a method to detect DNase II type DNA damage, bearing 5′OH using vaccinia topoisomerase I. The present invention also includes a method combining a ligase-based method to detect DNase I type DNA damage and the topoisomerase based method, resulting in simultaneous detection of two specific types of DNA damage in situ.
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