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Covalent Joining of DNA Strands to RNA Strands Catalyzed by Vaccinia Topoisomerase
Covalent Joining of DNA Strands to RNA Strands Catalyzed by Vaccinia Topoisomerase
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机译:Vaccinia Topoisomerase催化DNA链与RNA链的共价连接
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摘要
The present invention provides a method of covalently joining a DNA strand to an RNA strand, a method of tagging a 5′ end of an RNA molecule, a DNA-RNA molecule which has been joined in vitro by the use of a topoisomerase, a method of tagging a 5′ end of an mRNA, and a method of isolating and cloning full-length gene sequences using capped mRNA after subtraction of non-capped RNA.
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