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Covalent joining of DNA strands to RNA strands catalyzed by vaccinia topoisomerase
Covalent joining of DNA strands to RNA strands catalyzed by vaccinia topoisomerase
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机译:牛痘拓扑异构酶催化的DNA链与RNA链的共价连接
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摘要
The present invention provides a method of covalently joining a DNA strand to an RNA strand, a method of tagging a 5′ end of an RNA molecule, a DNA-RNA molecule which has been joined in vitro by the use of a topoisomerase, a method of tagging a 5′ end of an mRNA, and a method of isolating and cloning full-length gene sequences using capped mRNA after subtraction of non-capped RNA.
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