Plasmid shuttle vector between eschelichia coli and bacteria of genus brevibacillus

摘要

The invention provides a plasmid shuttle vector characterized by being replicable in both of bacteria of the genus Brevibacillus and Escherichia coli, and by comprising a promoter capable of functioning in bacteria of the genus Brevibacillus and a DNA sequence(s) encoding a sequence(s) capable of functioning as a selective marker(s) for Escherichia coli and bacteria of the genus Brevibacillus, and a method of transforming bacteria of the genus Brevibacillus using the said plasmid shuttle vector. ;The plasmid shuttle vector enables the efficient transformation of bacteria of the genus Brevibacillus. Further, since plasmid shuttle vector pNCMO2 of the invention in particular has a gene expression control region which efficiently expresses and secretes an objective gene in bacteria of the genus Brevibacillus but significantly suppresses the expression in Escherichia coli, a recombinant gene expression plasmid can efficiently be constructed using Escherichia coli, and it is suited for protein production in bacteria of the genus Brevibacillus.