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Tumor cells having tumorigenic potential but lacking invasive/metastatic potential, method for preparing them and screening method for metastasis-related genes using the same
Tumor cells having tumorigenic potential but lacking invasive/metastatic potential, method for preparing them and screening method for metastasis-related genes using the same
Tumor cells having tumorigenic potential but lacking invasive/metastatic potential are established by introducing an oncogene of the ras family into a BALB/c 3T3 A31-variant cell. A screening method for genes having the property of conferring invasive/metastatic potential is also provided, which comprises transfecting DNA derived from a tumor tissue obtained from the surface or inside of a mammal or derived from a tumor cell line into tumor cells having tumorigenic potential but lacking invasive/metastatic potential, isolating cells having acquired invasive/metastatic potential and extracting DNA therefrom.
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机译:通过将ras家族的癌基因引入BALB / c 3T3 A31变异细胞中,可以建立具有致癌潜力但缺乏侵袭/转移潜力的肿瘤细胞。还提供了一种具有赋予侵袭/转移潜力特性的基因的筛选方法,该方法包括将来源于从哺乳动物的表面或内部获得的肿瘤组织或来源于肿瘤细胞系的肿瘤组织的DNA转染到具有致瘤性但具有致瘤性的肿瘤细胞中。缺乏侵袭/转移潜力,分离具有侵袭/转移潜力的细胞并从中提取DNA。
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