Methods and apparatus for isolating invasive and metastatic cells for the evaluation of therapeutic agents and prediction of metastatic potential

摘要

Embodiments provide methods for producing assemblies that mimic in vitro the primary tumor site, secondary distant organs, and the circulating human microenvironment. Further provided are methods and devices for isolating primary tumors, invasive and metastatic cells and providing a platform for use in evaluation of therapeutic efficacy and diagnostics applied to metastatic cancer. In one aspect, a) a first component comprising a vertebrate-derived fluid and optionally a primary site growth matrix; b) a second component comprising a biological matrix mimic; and c) a secondary. A cell culture comprising a dynamic fluid component comprising a sex site growth medium, wherein the fluid component is in fluid contact with the first component and the second component An assembly is provided, wherein the secondary site growth medium has a higher serum concentration than the fluid.