Process for preparing cellulase and protease enzyme complex cud liquor from cattle and buffaloes cud for biodegradation of organic materials cud for biodegredation of organic materials

摘要

The present invention provides a process of preparing cellulase and protease enzyme complex cud liquor from cattle and buffaloes cud for biodegradation of organic materials. The process comprises feeding cattle/buffaloes green leguminous/non-leguminous fodder ad libitum. The fodder is withdrawn from said cattle/buffaloes after 2-3 hours and wait till cattle/buffalo is ruminating. Thereafter the fodder loop is inserted in the mouth of said cattle/buffaloes and said loop is allowed to stay in the mouth till the cud is embedded on said loop. The above steps are repeated with separate fodder loops and different cattle/buffaloes. The said loops are collected from the mouth of said cattle/buffaloes, and the loops embedded with cud are dipped in at least one litre buffer nutrient solution to obtain cellulase and protease concentrate.