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Grapevine fanleaf virus resistance in grapevine

机译:葡萄中的葡萄扇叶病毒抗性

摘要

In general, the invention features a method for selecting a transgenic grapevine or grapevine component having increased resistance to a grapevine fanleaf disease. The method involves (a) transforming a grape plant cell with a grapevine fanleaf virus coat protein nucleic acid molecule or fragment thereof which is expressed in the plant cell; (b) regenerating a transgenic grapevine or grapevine component from the plant cell; and (c) selecting a transgenic grapevine or grapevine component expressing a grapevine fanleaf virus coat protein or coat protein fragment thereof, where the coat protein or the coat protein fragment thereof is expressed at a level equal to or less than an optical density of about 0.2 at 405 nm as detected by an enzyme-linked immunoassay as compared to a control plant, the selected transgenic grapevine or grapevine component having resistance to the grapevine fanleaf disease.
机译:一般而言,本发明的特征在于选择对葡萄扇叶病具有增强的抗性的转基因葡萄或葡萄组分的方法。该方法包括:(a)用在植物细胞中表达的葡萄扇叶病毒外壳蛋白核酸分子或其片段转化葡萄植物细胞; (b)从植物细胞中再生出转基因葡萄或葡萄成分; (c)选择表达葡萄扇叶病毒外壳蛋白或其外壳蛋白片段的转基因葡萄或葡萄成分,其中外壳蛋白或其外壳蛋白片段的表达水平等于或小于约0.2的光密度。与对照植物相比,通过酶联免疫测定法在405nm处检测到,所选择的转基因葡萄或葡萄组分对葡萄扇叶病具有抗性。

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