Modified Cry Proteins sensitive to Pepsin; process to increase the sensitivity of The Cry Proteins to Pepsin, polynucleotides that encode such modified Proteins; Chimeric genes include such polynucleotides and vectors comprising the expression or processing

摘要

Describe and claim of Bacillus thuringiensis Cry Proteins, peptide Sequence modified so sensitized to specific enzymes in the digestive tract of mammals, particularly the pepsins. According to what is claimed, The Cry Proteins are modified by the insertion sites of the separation of the Pepsin, peptide sequences.The Modifications are preferred by the substitution of Aspartic acid residues with glutamic acid substitution remains, the remains of phenylalanine and tryptophan residue substitution of Valine or isoleucine residues with Leucine residue.A protein Protein Cry9C Cry is Preferred, and a favorite place to introduce the site separation of Pepsin in this Protein is the position 164. In addition, the processed Plant expressing these Proteins modified Cry and the remaining Objects mentioned in the title.Claim 2: Modified Cry Protein according to claim 1, wherein the additional site Cutting by Pepsin is represented by an elected among the remains of other amino acid phenylalanine or Leucine, glutamic acid.Claim 6: Cry Protein amended according to any of claims 1 to 5, characterized in that it has at least one additional site Cutting by Pepsin in at least one of the Inter Alpha Helixes loops of the I domain.