Cry3A modified toxins, polynucleotides encoding these Proteins, which include molecular constructs, host cells containing these constructsCompositions for controlling insects comprising Cry3A modified toxins and method to produce these Proteins.

摘要

Describes compositions and Methods for controlling plant pests.Provide, in particular, New Nucleic Acid sequences encoding modified toxins Cry3A have increased toxicity to the Corn Rootworm.By Inserting a protease Recognition site, such as cathepsin G, which is recognized by an intestinal protease of a Target Insect,In at least one position of a toxin is a toxin Cry3A, Designs modified Cry3A has significantly higher toxixidad,Particularly for Corn Rootworm in the West and North.In addition, it describes a new method to prepare Cry3A modified toxins and Methods of use of the frequency of Cry3A Nucleic Acid modifiedFor example, Transgenic insects or microorganisms to control implants to confer protection against damage caused by insectsAnd a method of using Cry3A modified toxins, and the compositions and formulations comprising Cry3A modified toxins,For example applying toxins or compositions or formulations of Cry3A modified areas infected by insects.To treat prophylactically or areas ssusceptibles insects or plants to confer protection against Insect pests.