Procedure for the identification of compounds that Alter receptor activity urotensina II, a Medicine and the use of compound obtained by this procedure to prepare a Medicine, useful for the therapy of the Ageing processes of the cardio Circulatory System

摘要

A procedure for the identification of compounds that modify the activity of the receptor urotensina II so that influences on the activity or amount of a Gene or Gene product involved in the Assembly or disassembly, formation of the Extracellular Matrix.For which: (a) provide a cell in which there is a recipient of the urotensina II; (b) provides a chemical compound; (c) the cell is in contact with the chemical compound (a) (b); (d) below determines the activity or amount of a Gene, or the Gene product of my We, from the cell (c), by that Gene or Gene product, in the Assembly,Training or disassembled, the Extracellular Matrix; e) Compares the activity or amount of (d) with the activity or amount of the same Gene or Gene product, on the basis of a corresponding control Cell, which has not been in contact with the compound (b). The cell is a cell of Mammal or human cell, for example a Heart Cell or Fibroblast.The chemical compound can be urotensina II In Step (d) select at least a Gene selected from the group consisting of Col 1, nnp2 TGFbeta1, or CTGF; in determining agreement with (d) or (e) takes place using quantitative PCR or occurs through consumption; Enzyme; Or takes place by an Antibody.Medicine containing a compound that has been identified by a procedure previously defined as also other substances that are necessary for the formulation of the drug for the treatment of a disease of the Circulatory System and the use of a compound iden Typhoid previously defined by the procedure for the preparation of a Medicine.