Method for modifying the Genome of a Guest, cassette of gene Recognition, Self replicating extrachromosomal Element and method of gene recognition

摘要

A method for modifying a Genome of a host.That includes the introduction of a cassette in the Genome of gene Recognition composed of: (a) a sequence of initiation of Replication in the Host by a factor known to mediate the replication of DNA Replication in the host initiated in the sequence of initiation of replicac Ion; (b) a reproducible Sequence linked to the Operational form of initiation of the replicaciu00f3 sequenceN so that DNA Replication is initiated in the sequence of initiation of Replication to replicate the reproducible sequence, to release a copy of the sequence reproducible; and,In which DNA Replication is initiated in the sequence of initiation of Replication results in the regeneration of the cassette of gene recognition for subsequent Cycles of DNA Replication to produce multiple copies of a reproducible Sequence; and in which at least one by These copies of a reproducible sequence of half an inheritable Genetic change in a Target sequenceCounterpart in the host Genome, Gene cassette of an extrachromosomal Self replicating Element Recognition, Recognition Method, and Gene. Claim 6: the method of claim 1, wherein the host, or a relative linear host, is transformed into a sequence of nucleotides encoding the Replication factor.Claim 7: Method of claim 6, wherein the Nucleotide sequence encoding the Replication factor is expressed under the control of a promoter selected from the group consisting of specific promoters of Cell Cycle Promoters specific for F Ase G1 s Phase specific Promoters, the promoters of the G1 / s Boundary, tissue specific Promoters.Promoters of Development Stage specific Promoters in response to Environmental Stimuli, constitutive Promoters, developers, bipartite, or Promoters that are regulated by Induction or repression. Claim 8: the method of claim 1, wherein the host is e Ucariu00f3tico and a replication factor includes a nuclear localization sequence.Claim 16: the method of claim 1, wherein the host is a Plant Cell or Plant. Claim 17: the method of claim 1, wherein the host is an animal Cell or an animal.Claim 18: the method of claim 1, characterized in that it includes the step of removing the recognition of gene cassette in the genome.