Method, kit and primers for the idendificau00e7u00e3o sequences specific nucleotu00ecdeos through the chain reaction of polimeras type nested in a single tube reaction

摘要

Method, kit and primers for the identification of sequences especu00ecficas of nucleotu00ecdeos by polymerase chain reaction type nested in a single tube reaction.The objective of this u00ednvenu00e7u00e3o is to eliminate the risk of cross contamination of reacionais mixtures to be used in the two stages of the polymerase chain reaction type nested by a methodsThe simple and effective period, where there is no commitment of the specificity and sensitivity of the system and the amplification and identification of the sequ00b3u00eancia specific nucleotides.The method is characterized by nested PCR in a single tube type reaction, in which the internal primers are fixed, by evaporation.On the inner side of the lid of the tube until the end of the first phase of amplification, when is promoted its contact with the reactional mixture and, therefore, begins the second stage of amplification.In addition, the invention relates to a kit for use in the amplification and identification of sequ00b3u00eancias nucleotide specific human beings, plants,Animals and other single celled organisms and pluricelulares. The invention also includes primers specific for Plasmodium sp. and Schistosoma sp.