INTEGRATED RT-PCR/NESTED PCR DIAGNOSTICAL SYSTEM FOR DETECTING PLUM POX VIRUS AND DIFFERENTIATING SUBGROUPS THEREOF

摘要

the object of the invention is an integrated u201enested "pcr / rt-pcr procedure aszilvahimlu0151vu00edrus (u201eplum potyvirus pox", i.e., detection of dideron u00e9sfontosabb ppv), (d), marcus (m), el amar (e) and sour cherries (soc), to identify the next to each other.the overall ppv detection of rt - pcr, the ku00f6penyfehu00e9rje (cp) - n - terminal part of ku00f3dolu00f3gu00e9nszakaszt ku00f6zrefogu00f3 lu00e1ncindu00edtu00f3kalkalmazu00e1su00e1val degenerate sequences. the pcr product was specifikusszekvenciaeltu00e9ru00e9sek are located within subtypes.the second step of the procedure, u201enested "pcr is carried out in the first pcr product, the subtypes of specifikusszekvenciu00e1khoz hibridizu00e1lu00f3du00f3 lu00e1ncindu00edtu00f3k using pcr. atalu00e1lmu00e1ny are the subject of the invention in accordance with the procedure alkalmazottoligonukleotid lu00e1ncindu00edtu00f3 couples too.according to the invention megoldu00e1salkalmas plant material ppv - contamination detection, in the case of u00e9sfertu0151zu00f6ttsu00e9g infectious virus subtype identification. a