PHOSPHINOTHRICIN RESISTANCE GENE OBTAINABLE FROM STREPTOMYCES VIRIDOCHROMOGENES AND ITS USE

摘要

Phosphinothricin (PTC) - resistance gene obtainable by selecting streptomyces viridochromogenes DSM 4112 for resistance to phosphinothricyl-alanyl- alanine (PTT), cutting with BamHI the total DNA from the resistant strains, cloning a fragment 4.0 kb in size, retransformation into sensitive S. lividans and selecting for PTT resistance.