A reproducible xenograft animal model for hosting and propagating human papillomavirus (HPV)

摘要

Described is a graft animal model for propagating papillomavirus and for evaluating and testing candidate therapeutic agents against papillomavirus. The animal model comprises a recipient animal engrafted with injured skin graft infected with a host-specific papillomavirus (PV). The grafted skin, having demonstrable papillomas supports the propagation of its host-specific PV. Also described is a xenograft animal model for hosting and propagating human papillomavirus (HPV), thereby providing a means for generating infectious and passaging HPV suspensions, and for screening candidate therapeutic agents against HPV. In addition, a method for generating the xenograft human animal model is described also. The graph depicts single type HPV-11 wart induction in the cutaneous model by viral stock originally generated from sub-cutaneous xenograft warts. It demonstrates the high frequency of papilloma induction with single type HPV-11 in 2nd and 3rd generation using papillomavirus collected from serial xenograft passage.