METHOD AND SYSTEM DESIGNED TO INCREASE SENSITIVITY OF MASS SPECTROMETER WITH GASEOUS ION SOURCE AND THERMIONIC CATHODE

• 1. 一种提高飞行时间质谱仪灵敏度的方法 [P] . 中国专利： CN109103067B . 2020.01.17
• 2. 一种提高飞行时间质谱仪灵敏度的方法 [P] . 中国专利： CN109103067A . 2018-12-28
• 3. Method and system designed to increase sensitivity of mass spectrometer with gaseous ion source and thermionic cathode [P] . PL201623B1 . 2009-04-30

  机译： 设计用于提高具有气态离子源和热离子阴极的质谱仪灵敏度的方法和系统

• 4. OPTIMUM DESIGN OF POST GENOME MASS SPECTROMETER FOR INCREASING SENSITIVITY AND MINIATURIZATION (RIGHT ANGLE SPRAY IONIZATION TYPE) [P] . 日本专利： JP2006073490A . 2006-03-16

  机译： 用于提高灵敏度和最小化的后基因组质谱仪的优化设计（直角喷雾电离类型）

• 5. Method of increasing the dynamic range and sensitivity of a quadrupole ion trap mass spectrometer operating in the chemical ionization mode [P] . 美国专利： US4771172A . 1988-09-13

  机译： 增加以化学电离模式运行的四极离子阱质谱仪的动态范围和灵敏度的方法

• 1. NEW METHOD FOR MEASURING SECONDARY ELECTRON EMISSION OF THERMIONIC CATHODE AND ITS APPLICATION [J] . 陈德森, 孙学民 电子科学学刊：英文版 . 1990,第003期
• 2. Study of Emission Character of High Current Densities Thermionic Cathodes for Electron Beam Processing System [J] . Cao Shuchun, Li Zhongping, Zhang Ziming, 近代物理研究所和兰州重离子加速器实验室年报：英文版 . 2008,第001期
• 3. 6-23 Design of Vacuum System for Magnetic Mass Spectrometer [J] . Xie, Wenjun, Meng, 中国科学院近代物理研究所和兰州重离子研究装置年报：英文版 . 2015,第001期
• 4. A MALDI/C_(60) SIMS dual ion source Q-TOF mass spectrometer for chemical imaging: Design and system characterization [C] . Eric J. Lanni, Peter Nemes, Ta-Hsuan Ong, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2012
• 5. Sub-miniature double-focusing mass spectrometer for in situ environmental monitoring and application to volcanic gaseous emissions. [D] . Diaz Diaz, Jorge Andres. 1999
• 6. Design of Portable Mass Spectrometers with Handheld Probes: Aspects of the Sampling and Miniature Pumping Systems [O] . Chien-Hsun Chen, Tsung-Chi Chen, Xiaoyu Zhou, -1
• 7. A method of increasing the sensitivity for laser desorption in an ion trap mass spectrometer [O] . Eiden G.C., Cisper M.E., Alexander M.L., 1993

• 1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 2. Determination of phytosterols in iOryza sativa/iL. cultivars by liquid chromatography atmospheric chemical ionization time-of-flight mass spectrometer (LC-APCI-TOF-MS) coupled with a modified QuEChERS method [J] . Seung Rok Lee ,Dong Cheon Yun ,Seung Ho Lee . 农业化学和环境（英文） . 2012,第1期
• 3. 便携式双离子源飞行时间质谱仪及其选择性离子去除方法的研究 [C] . HUANG Ru-jun ,黄如俊 ,HE jian . 中国质谱学会第32届年会 . 2012
• 4. 提高真空环境中痕量杂质四极质谱仪灵敏度的实验分析方法研究 [A] . 杨玲玲 . 2015