CRYOPRESERVATION MEDIUM FOR PRIMATE EMBRYO STEM CELLS AND CRYOPRESERVATION METHOD

摘要

ABSTRACT A cryopreservation medium and a cryopreservationmethod which make it possible to cryopreserve ES cells from primates simply with high viability are provided. A cryopreservation medium containing acryoprotectant at a concentration of from 12% (W/V) to 50% (W/V) and a cryopreservation method for primate embryonic stem cells, which comprises a step of suspending primate embryonic stem cells in thecryopreservation medium, and a refrigeration step of freezing the suspension of the primate embryonic stem cells in the cryopreservation medium by cooling it to -80[err]C or below at a rate of from 0.5[err]C to 10[err]C per minute,and a preservation step of storing the frozen suspension of primate embryonic stem cells in the cryopreservation medium enable simple cryopreservation of primateembryonic stem cells with high viability.