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METHOD FOR PRODUCING CELL LINES AND ORGANS BY MEANS OF DIFFERENTIABLE CELLS
METHOD FOR PRODUCING CELL LINES AND ORGANS BY MEANS OF DIFFERENTIABLE CELLS
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机译:用不同细胞制备细胞系和器官的方法
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摘要
The invention relates to a method for the production of cell lines or individual organs, whereby differentiable donor cells are supplied to a morula or a blastocyst cultivated under conditions which enable further development of the morula or blastocyst in stages in which newly formed cell lines with a high degree of differentiation are produced, and isolation of said cell lines or further differentiation of said cell lines in organs. The inventive method is characterised in that the cells of the morula or the inner cellular mass of the blastocyst have a limited survival capacity compared to the respective wild type, or their survival capacity is reduced by suitable conditions of cultivation, and the donor cells supplied to the morula or the blastocyst have different degrees of differentiation. Compared to conventional methods, the inventive method enables the cultivation period of the donor cells to be essentially reduced, favouring, or at least enabling, the production of natural stem cells. Another use of the blastocyst involves transferring the same into a surrogate animal.
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