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METHOD FOR DISCOVERING SUITABLE CHROMATOGRAPHIC CONDITIONS FOR SEPARATING BIOLOGICAL MOLECULES
METHOD FOR DISCOVERING SUITABLE CHROMATOGRAPHIC CONDITIONS FOR SEPARATING BIOLOGICAL MOLECULES
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机译:发现用于分离生物分子的合适色谱条件的方法
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摘要
The invention relates to a multiparallel chromatographic system for developing methods for purifying proteins and other biomolecules. Said system uses cavities of multi-well plates (e.g. 96-well plates) which are filled with chromatographic gels, and consists of various starting buffers in which the samples to be chromatographed are dissolved and by which means the gels are equilibrated, and solutions for desorbing (eluting) the biomolecules bonded to the gels. Said system comprises aids for interpreting the results which are deduced from the analyses (e.g. protein concentration determinations, bioassays etc.), following the chromatography, of the supernatants of the non-bonded biomolecules and/or the eluates. Said aids can consist of programmes into which the results are inputted and which, once the results have been processed, supply interpretations and suggestions for creating the steps for purifying a biomolecule.
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