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Method for discovering suitable chromatography conditions for the separation of biological molecules

机译:发现合适的色谱条件以分离生物分子的方法

摘要

The invention relates to a multiparallel chromatography system for developing a method for the purification of proteins and other biomolecules. The system uses cavities of multiwell plates (for example 96 well plates) filled with chromatography gels, and consists of diverse start buffers in which the samples to be chromatographed are dissolved and with which the gels are equilibrated, and solutions for desorption (elution) of the biomolecules bound to the gels. The system comprises aids for interpretation of the results obtained from the post-chromatography analyses (for example protein concentration determinations, bioassays, etc.) of the supernatants of the unbound biomolecules and/or the eluates. These aids may also consist of programs into which the results are entered and which, after processing of the results, supply interpretations and proposals for the design of the steps for the purification of a biomolecule.
机译:本发明涉及用于开发纯化蛋白质和其他生物分子的方法的多平行色谱系统。该系统使用装有层析凝胶的多孔板(例如96孔板)的腔体,并由多种起始缓冲液组成,将要色谱分析的样品溶解在该缓冲液中,并使凝胶平衡,并用溶液进行解吸(洗脱)与分子结合的生物分子。该系统包括用于解释从未结合的生物分子和/或洗脱液的上清液的色谱后分析(例如蛋白质浓度测定,生物测定等)获得的结果的辅助工具。这些辅助工具还可以包括输入结果的程序,在处理结果之后,提供解释和建议,以设计生物分子的纯化步骤。

著录项

  • 公开/公告号US2006096924A1

    专利类型

  • 公开/公告日2006-05-11

    原文格式PDF

  • 申请/专利权人 HARTMUT SCHLUETER;

    申请/专利号US20050528103

  • 发明设计人 HARTMUT SCHLUETER;

    申请日2003-09-19

  • 分类号B01D15/08;

  • 国家 US

  • 入库时间 2022-08-21 21:48:18

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