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SOL-GEL OPEN TUBULAR ODS COLUMNS WITH CHARGED INNER SURFACE FOR CAPILLARY ELECTROCHROMATOGRAPHY
SOL-GEL OPEN TUBULAR ODS COLUMNS WITH CHARGED INNER SURFACE FOR CAPILLARY ELECTROCHROMATOGRAPHY
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机译:带电荷的内表面的SOL-GEL空心管状ODS色谱柱,用于毛细管电色谱
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摘要
The present invention provides for a column for use in capillary electrochromatography including a tube (12), wherein the tube (12) includes an inner surface (20) having a positively or negatively charged, chemically-bonded stationary coating (14) thereon. The present invention further provides for a method for making the tube, wherein the steps include filling the tube with a sol-gel solution, maintenaing the sol-gel solution whitin the tube, and forcing the sol-gel solution out of the tube with inert gas. Further, the present invention provides for a method of analytical separation with a column including an inner layer having a positively charged chemically-bonded stationary coating thereon that reverses the direction of electroosmotic flow in the column compared with a column without the positive surface charge.
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