METHOD OF IDENTIFYING PANCREATIC DUCTAL CARCINOMA-SPECIFIC GENE USING PANCREATIC DUCTAL CELLS, METHOD OF TESTING FOR PDC USING SAID GENES, AND METHOD OF SCREENING PHARMACEUTICAL CANDIDATE COMPOUNDS FOR TREATING OR PREVENTING PDC

摘要

We purified ductal epithelial cells, by the use of affinity column for MUC1, from the pancreatic juice isolated from healthy individuals as well as those with PDC. Microarray analysis among these background-matched samples of 3456 human genes has identified a number of carcinoma-specific genes, such as those for AC133, CEACAM7 and SOD2. Cancer-specific expression of these genes was further confirmed by a quantitative real-time PCR method. Our microarray analysis with purified pancreatic ductal cells has paved a novel way to develop a sensitive detection method for PDC by the use of pancreatic juice which is routinely obtained in clinical conditions. A pancreatic ductal carcinoma-specific gene can be efficiently identified by utilizing this method, and thereby, it is possible to provide a target that is important for developing a drug for the test of pancreatic ductal carcinoma and the treatment or prevention of pancreatic ductal carcinoma.