Novel strains of yeast of genus- saccharomyces species- cerevisiae and a process for the preparation of such strains of yeast

摘要

The present invention relates to a novel strain of yeast Saccharomyces cerevisiae having the accession number MTCC Y0022B211 (=NCYC 2647) which is useful for the preparation of ethanol by the fermentation of sugars and a process for the preparation of said strain of yeast, which process comprises, (a) growing a diploid strain of yeast Saccharomyces cerevisiae designated as MTCC Y0001 (=NCYC 2646) in a known medium, sporulating the strain, treating the sporulated cells with a known lytic enzyme, collecting the liberated spores, (b) growing a haploid strain of yeast Saccharomyces cerevisiae designated as MTCC Y0002 (=ATTC 90506), (c) mixing the spores obtained in step (a) with the cells obtained in step (b), incubating the resultant spore-cell mixture at a temperature in the range of 15 to 37 DEG C for a period of 1 to 10 days, (d) spreading the incubated spore-cell mixture over a known non-selective medium, and incubating the said mixture at a temperature in the range of 15 to 37 DEG C for a period of 1 to 10 days, and (e) collecting the cells produced in step(d), and spreading over a selective medium so as to eliminate the spores/cells of steps (a) and (b) and allowing only hybrid cells/cytoductants to grow.