TROPISM-MODIFIED ADENOVIRAL VECTORS, PREFERABLY FOR TARGETING B-LYMPHOCYTES OR OVARIAN CELLS

摘要

The invention concerns recombinant adenoviruses whereof the tropism has been modified to enable B-lymphocytes or ovarian cells to be infected thereby. The inventive recombinant adenoviruses exhibit a reduced tropism compared to that of the native adenovirus. The invention also concerns methods for preparing said adenoviruses, as well as their uses, in particular for transferring DNA sequences into B-lymphocytes or ovarian cells in vitro, ex vivo or in vivo, for experimental, industrial, vaccination or therapeutic purposes. The invention more specifically concerns the modification of the gene of the adenovirus fiber for targeting B-lymphocytes or ovarian cells, in particular tumor cells, as well as other modifications and/or constructs enabling the viruses to be provided with a character specific to said cells. The invention is particularly characterized either by insertion into the fiber of the sequence of a peptide identifying a receptor expressed at the surface of B-lymphocytes, or by replacing the Mastadenovirus adenovirus fiber bud with a fiber bud of a phylogenetically distant adenovirus, preferably of the bovine adenovirus serotype 4 (BAV4).