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ORGANISM FINGERPRINTING USING NICKING AGENTS
ORGANISM FINGERPRINTING USING NICKING AGENTS
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机译:使用网民代理的有机指纹
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摘要
A sample of nucleic acid that is characteristic of a particular source, e.g., a bacterium, can be analyzed to determine whether that particular sample is from a particular source. The analysis protocol subjects the sample to a nicking reaction to produce a family of nicked fragments. In certain embodiments, the nicking reaction is performed in the presence of a polymerase and nucleoside triphosphates to amplify nicked fragments or portions thereof. The nicked fragments or portions thereof may be analyzed to determine the source of the nucleic acid sample.
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