DNA FRAGMENT AMPLIFICATION METHOD, REACTION APPARATUS FOR AMPLIFYING DNA FRAGMENT AND PROCESS FOR PRODUCING THE SAME

摘要

A reaction apparatus (10) having a base plate (12) and a plurality of columns (14) formed on the base plate (12). To the surface of the base plate (12) and the columns (14), an oligonucleotide for immobilization (16) having a sequence complementary to the both terminals of an initial template DNA (18) is bonded. By transferring the initial template DNA (18) in the extended state, the initial template DNA (18) can be immobilized while straddling columns (14) adjacent to each other. PCR is carried out in this state.