DNA FRAGMENT AMPLIFICATION METHOD, REACTION APPARATUS FOR AMPLIFYING DNA FRAGMENT AND PROCESS FOR PRODUCING SAME

摘要

A reaction apparatus (10) includes a substrate (12) and a plurality of columnar members (14) formed on the substrate (12). Oligonucleotides for immobilization (16) having sequences complementary to sequences of both ends of a starting template DNA (18) is adhered on the surfaces of the substrate (12) and the columnar members (14). The starting template DNA (18) can be immobilized over the adjacent columnar members (14) by introducing the starting template DNA (18) under the elongated condition. PCR is conducted in such condition.