Isolation and purification of hydrophobin protein from Tricholoma matsutake including useful tradition filamentous fungi its gene sequence and a composition containing the same

摘要

PURPOSE: An isolation and purification method of hydrophobin protein from Tricholoma matsutake including useful tradition filamentous fungi, a gene sequence thereof and a composition containing the protein and gene sequence are provided. The hydrophobin has hydrophilic and hydrophobic converting properties, so that it can be useful in cosmetic industry, brewing industry and medicine industry. CONSTITUTION: An isolation and purification method of hydrophobin protein from Tricholoma matsutake including useful tradition filamentous fungi comprises the steps of: adding 60% ethanol into a fruit body of Tricholoma matsutake, pulverizing it, and centrifuging the pulverized solution; adding distilled water into the supernatant and subjecting the mixture to dialysis for overnight; centrifuging the remaining solution at 4 deg. C and freeze-drying the supernatant; dissolving the freeze-dried substance in 25 ml of 0.05M sodium-phosphate buffer solution(pH 6.0) and 1M sodium solution and mixing them; centrifuging the mixture at room temperature; and washing the precipitated hydrophobin containing portion with 1M sodium buffer solution, 80% ethanol and a mixed solution of chloroform and methanol, and drying it. A gene encoding the hydrophobin protein from Tricholoma matsutake has the nucleotide sequence set forth in SEQ ID NO: 4.