Preparation of hybridomas producing monoclonal antibodies against human Fas-associated factor 1 and their application for the immunoprecipitation of hFAF1

摘要

PURPOSE: Monoclonal antibodies against human Fas-associated factor 1 (hFAF1) and hybridomas producing the same are provided, thereby specifically detecting FAF1 protein and inducing immunoprecipitation of hFAF1, so that they can be used in quantification of cell death-associated proteins including Fas protein and verification of association of these proteins to cell death diseases. CONSTITUTION: The monoclonal antibodies against human Fas-associated factor 1 (hFAF1) are derived from mouse, and produced from hybridomas FAF-85(KCTC 10366BP) and FAF-99(KCTC 10367BP). A method for producing the hybridomas producing the monoclonal antibodies against human Fas-associated factor 1 (hFAF1) comprises the steps of: immunizing a mouse with a GST(Glutathion-S-transferase) fusion protein of human Fas-associated factor 1 (hFAF1); fusing spleen cells of the immunized mouse with myeloma cell; and culturing the fusion cells to produce the monoclonal antibodies against human Fas-associated factor 1 (hFAF1). The method for diagnosis of cell death-associated diseases comprises measuring the expression level of human Fas-associated factor 1 (hFAF1) using the monoclonal antibodies against human Fas-associated factor 1 (hFAF1) produced from hybridomas FAF-85(KCTC 10366BP) or FAF-99(KCTC 10367BP).