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hTERT PROMOTER WITH ENHANCED TUMOR-SPECIFICITY AND STRENGTH AND RECOMBINANT VECTOR COMPRISING THE SAME TO SELECTIVELY KILL CANCER CELLS
hTERT PROMOTER WITH ENHANCED TUMOR-SPECIFICITY AND STRENGTH AND RECOMBINANT VECTOR COMPRISING THE SAME TO SELECTIVELY KILL CANCER CELLS
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机译:具有增强的肿瘤特异性和强度的hTERT启动子,以及重组载体,包括相同的选择性杀伤癌细胞
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PURPOSE: An hTERT(human telomere reverse transcriptase) promoter with enhanced tumor-specificity and strength and a recombinant vector comprising the same are provided, thereby specifically and highly expressing the hTERT gene in only cancer cells. Therefore, cancer cells can be selectively killed. CONSTITUTION: A transcription regulating sequence comprises the hTERT promoter and one or more of c-Myc binding sites and/or one or more of Sp1 binding sites which are linked to the hTERT promoter, wherein the c-Myc binding site has the nucleotide sequence selected from SEQ ID NO: 2, SEQ ID NO:3 and SEQ ID NO:4; the Sp1 binding site has the nucleotide sequence selected from SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10 and SEQ ID NO:11; the regulating sequence has the nucleotide sequence set forth in SEQ ID NO:14. The recombinant vector comprises the transcription regulating sequence which is operably linked to the gene required for virus replication, wherein the virus vector is derived from Adenoviridiae; the gene required for virus replication is an initial gene E1A.
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机译:目的:提供具有增强的肿瘤特异性和强度的hTERT(人端粒逆转录酶)启动子和包含该启动子的重组载体,从而仅在癌细胞中特异性和高表达hTERT基因。因此,可以选择性地杀死癌细胞。组成:转录调控序列包含hTERT启动子和与hTERT启动子连接的一个或多个c-Myc结合位点和/或一个或多个Sp1结合位点,其中c-Myc结合位点具有选择的核苷酸序列SEQ ID NO:2,SEQ ID NO:3和SEQ ID NO:4; Sp1结合位点具有选自SEQ ID NO:5,SEQ ID NO:6,SEQ ID NO:7,SEQ ID NO:8,SEQ ID NO:9,SEQ ID NO:10和SEQ ID NO:的核苷酸序列。 11;调节序列具有SEQ ID NO:14所示的核苷酸序列。重组载体包含与病毒复制所需的基因可操作连接的转录调节序列,其中病毒载体来源于腺病毒科。病毒复制所需的基因是初始基因E1A。
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