Inclusion body in the form of miliary inchegwa leukocyte reproducing method of colony stimulating factor with a gel filtration column chromatography

摘要

PURPOSE: A process for reproducing a human granule-colony stimulating factor(hG-CSF) is provided which can reduce a reaction volume and obtain a high yield of the hG-CSF by using an inclusion body of the hG-CSF, so it is easy to produce the hG-CSF in a great quantity CONSTITUTION: The process comprises the steps of: suspending the inclusion body of the hG-CSF expressed from a recombinant strain which is cultivated in a solution containing DL-Dithiotheritol(DTT) and sodium dodecyl sulfate(SDS); adjusting pH of the culture solution to an alkali condition; passing the culture solution through a gel filtration column chromatography equilibrated with a buffer solution containing SDS and Cu¬2+; and recovering a fraction of the reproducing hG-CSF.