PROCESS FOR THE PURIFICATION OF RECOMBINANT HUMAN GRANULOCYTE-COLONY STIMULATING FACTOR IN THE FORM OF INCLUSION BODY FROM YEAST

摘要

The present invention relates to a method for purifying recombinant phosphorus granulocyte colony stimulator expressed in yeast, and to obtain the inclusion body by crushing yeast cells expressed phosphorus granulocyte colony stimulating factor (rhG-CSF) as inclusion bodies, and dissolve the inclusion body The oxidized inclusion body was oxidized to form disulfide bonds, rhG-CSF with disulfide bonds formed was precipitated, separated, and redissolved to perform cation exchange chromatography, hydrophobic chromatography, and gel filtration chromatography. G-CSF having can be purified in high yield.