Plant cDNA GCYC1, which endows high temperature stress resistance in bacteria or eukaryotic cells

摘要

The present invention relates to a method for producing a bacterial cell and has a high temperature resistance by introducing a high-temperature-resistant gene (heat-resistant gene), the high temperature resistance gene isolated from the specific non-single why jalrokyi (Griffithsia japonica)GCYC1; This high expression E. coli strain; Method for preparing the bacteria or cells with a high temperature resistance by introducing the gene; And it relates to a method for preventing the denaturation of the protein at high temperature conditions using the recombinant protein expressed from the gene. Since high-temperature-resistant gene GCYC1 of the present invention inhibits the growth temperature range, the protein of the small bacteria, and cells, such as E. coli that cause denaturation at high temperatures, and to increase the survival rate by giving resistance to high temperature, transfection of industrially useful microorganisms may be useful, such as transition or a high temperature is required in the reaction solution which enzyme reaction, etc. prevent protein denaturation.