PURPOSE: A method for preparing mitogen-activated protein kinase kinase-1(MAPKK-1 or MEK-1) with high purity is provided, which protein MEK-1 is useful for analysis of mutual relation between protooncogene such as ras and cancer, so that it can be useful for prevention and treatment of cancer. CONSTITUTION: The method for preparing mitogen-activated protein kinase kinase-1(MAPKK-1 or MEK-1) with high purity comprises the steps of: preparing MEK-1 gene in which the nucleotide sequence encoding amino acids at amino acid position 33 to 52 are deleted, the nucleotide sequence encoding an amino acid at amino acid position 222, serine is substituted by aspartic acid, the nucleotide sequence encoding histidine set forth in SEQ ID NO:1 consisting of 5'-ATG CAT CAT CAC CAC CAT CAC GGA TCC CAT ATG CCC AAG AAG AAG CCG-3' is present in the 5'-terminal of the nucleotide sequence; constructing an expression vector pBacHis MEK-1 containing the MEK-1 gene; transforming Baculovirus with the expression vector pBacHis MEK-1 to produce Baculovirus AcMNPV MEK-1(KCTC 0287BP); and culturing Baculovirus AcMNPV MEK-1(KCTC 0287BP) and purifying protein MEK-1 from the cultured Baculovirus cells.
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