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A Diagnostic Kit for Male Sterility and a Method for Detecting Deletion of Male Sterility-related DNA Using the Same

机译:男性不育诊断试剂盒和使用该方法检测男性不育相关DNA的缺失的方法

摘要

PURPOSE: A mail sterility diagnosis kit and a method for analyzing a result of DNA(Deoxyribonucleic Acid) related to sterility of the male using the mail sterility diagnosis kit are provided to improve the accuracy and the speed of analysis by measuring a ferment activity and a fluorescent of material. CONSTITUTION: A solid support is adhered to a probe needle including a base sequence of a primer for amplifying DNA related to a sterility of the male. A PCR(Polymerase Chain Reaction) mixture is comprised of the primer for amplifying the DNA related to sterility of the male, dNTP, DNA polymerism ferment, and a buffer solution. An additional primer is comprised of fifteen to thirty bases and coupled to a biotin at 5'-end thereof. A strapped avidin and a substrate of an electric labeled ferment are coupled to a labeled ferment.
机译:目的:提供一种邮件无菌性诊断试剂盒以及使用该邮件无菌性诊断试剂盒分析与男性无菌性有关的DNA(脱氧核糖核酸)结果的方法,以通过测量发酵活性和酶促分析提高准确性和分析速度。材料的荧光。构成:将固相支持物粘附到探针上,该探针包含用于扩增与雄性不育有关的DNA的引物的碱基序列。 PCR(聚合酶链反应)混合物由用于扩增与雄性不育相关的DNA的引物,dNTP,DNA聚合发酵液和缓冲液组成。额外的引物由十五至三十个碱基组成,并在其5'末端与生物素偶联。绑扎的抗生物素蛋白和电标记的发酵物的底物偶联至标记的发酵物。

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