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Diagnostic methods for male infertility and diagnostic kits for male infertility for detecting Y chromosomal microdeletion
Diagnostic methods for male infertility and diagnostic kits for male infertility for detecting Y chromosomal microdeletion
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机译:男性不育的诊断方法和检测Y染色体微缺失的男性不育的诊断试剂盒
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摘要
An analysis method and a diagnostic kit are provided to solve a problem of being unable to determine a deleted portion using an STS marker by diagnosing microdeletion of Y chromosome regarding genes on the Y chromosome, determine the microdeletion of a specific gene generated from each of infertile patients in a molecular biological level and analyze a large amount of sample conveniently, accurately and rapidly by detecting the microdeletion of various genes located at Yp, AZFa, AZFb, and AZFc of the Y chromosome through multiplex-polymerase chain reaction(M-PCR). An analysis method for providing information required for diagnosing male infertility comprises a step of detecting microdeletion of at least one gene in a gDNA sample isolated from a male patient and selected from the group consisting of TMSB4Y, AMELY, PS4Y2, VCY, ZFY, TBL1Y, SMCY, XKRY, CYorf15A, NLGN4Y, EIF1AY, UTY, RBM1, CYorf15B, TTTY5, TTTY6, TTTY17, and DDX3Y, wherein the gDNA sample is isolated from blood of the male patient and the detection is performed through polymerase chain reaction using at least one primer pair. A diagnostic kit of the male infertility for detecting the microdeletion of the gene in the gDNA sample isolated from the male patient comprises a primer pair capable of amplifying at least one of the gene selected from the group consisting of TMSB4Y, AMELY, RPS4Y2, VCY, ZFY, TBL1Y, SMCY, XKRY, CYorf15A, NLGN4Y, EIF1AY, UTY, RBM1, CYorf15B, TTTY5, TTTY6, TTTY17, and DDX3Y.
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