Y chromosome Male infertility diagnostic kit for the detection Microdeletion

摘要

A diagnostic method and a diagnostic kit are provided to determine microdeletion of a specific gene generated from each of infertile patients in a molecular biological level and analyze a large amount of sample conveniently, accurately and rapidly by detecting the microdeletion of various genes located at Yp, AZFa, AZFb, and AZFc of the Y chromosome through multiplex-polymerase chain reaction(M-PCR). A method for diagnosing male infertility comprises a step of detecting microdeletion of at least one gene in a gDNA sample isolated from a male patient and selected from the group consisting of TMSB4Y, AMELY, RPS4Y2, VCY, ZFY, TBL1Y, SMCY, XKRY, CYorf15A, NLGN4Y, EIF1AY, UTY, RBM1, CYorf15B, TTTY5, TTTY6, TTTY17, and DDX3Y, wherein the gDNA sample is isolated from blood of the male patient, the detection is performed through polymerase chain reaction using at least one primer pair and the primer pair is at least one selected from the group consisting of SEQ ID : NOs. 3 and 4; SEQ ID : NOs. 3 and 4; SEQ ID : NOs. 9 and 10; SEQ ID : NOs. 11 and 12; SEQ ID : NOs. 13 and 14; SEQ ID : NOs. 17 and 18; SEQ ID : NOs. 19 and 20; SEQ ID : NOs. 21 and 22; SEQ ID : NOs. 23 and 24; SEQ ID : NOs. 25 and 26; SEQ ID : NOs. 27 and 28; SEQ ID : NOs. 29 and 30; SEQ ID : NOs. 31 and 32; SEQ ID : NOs. 33 and 34; SEQ ID : NOs. 35 and 36; SEQ ID : NOs. 37 and 38; SEQ ID : NOs. 39 and 40; and SEQ ID : NOs. 41. A diagnostic kit of the male infertility for detecting the microdeletion of the gene in the gDNA sample isolated from the male patient comprises a primer pair capable of amplifying at least one of the gene selected from the group consisting of TMSB4Y, AMELY, RPS4Y2, VCY, ZFY, TBL1Y, SMCY, XKRY, CYorf15A, NLGN4Y, EIF1AY, UTY, RBM1, CYorf15B, TTTY5, TTTY6, TTTY17, and DDX3Y.