SIGNAL SEQUENCE FOR OBTAINING LEU-HIRUDIN BY SECRET USING E.COLI IN A CULTURAL ENVIRONMENT

摘要

The present invention relates to a efficient expression of a protein, particularly, hirudin; a protein precursor comprising a signal sequence and the sequence of aaSUBX/SUB-hirudin, wherein aaSUBX /SUBis a selected amino acid, and aaSUBX /SUBis preferably leucine; to its preparation and use; and to processes for finding signal sequences for secretory expression of any desired protein in E coli; and to processes for the secretory expression of any desired protein in E coli.