. - Signal sequences for the production of Leu-hirudin via secretion by E.coli in a culture medium

摘要

The present invention also relates to a method for the detection of a signal sequence for secretory expression of a hirudin precursor comprising a signal sequence and secretion expression of such Leu-hirudin and any desired protein in E. coli , And methods for secretory expression of any desired protein in E. coli.