RECOMBINANT PLASMID DNA pBMC-nef(A)m-1 EXPRESSING PROTEIN NEF OF HUMAN IMMUNODEFICIENCY VIRUS TYPE I VIRUS IN EUCARYOTIC CELLS

摘要

FIELD: biotechnology, medicine, molecular biology, genetic engineering, immunology. SUBSTANCE: invention proposes recombinant plasmid DNA pBMC-nef(A)m-1 that comprises gene nef of human immunodeficiency virus type I, subtype A, where in its sequence of nucleotides in codons with prevailing adenine and/or thymine bases adenine and/or thymine are replaced partially for guanine and/or cytosine. Plasmid provides the expression of protein NEF and can be used for immunization against human immunodeficiency virus. EFFECT: valuable biological and medicinal properties of plasmid DNA. 1 tbl, 3 dwg