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Amplifying information from genetic material containing many fragments, useful e.g. for analyzing frequency of chromosomes in oocytes

机译:从包含许多片段的遗传材料中扩增信息,例如用于分析卵母细胞中的染色体频率

摘要

Amplifying genetic information from genetic material (B) that contains many fragments (B') of material, separated from each other, comprising using PCR with primers (P) that are complementary to binding sites present at many locations in (B), is new. Amplifying genetic information from genetic material (B) that contains many fragments (B') of material, separated from each other, by using PCR with primers (P) that are complementary to binding sites present at many loctions in (B). Each binding site is adjacent to one (B') of the target sequence, of predetermined length, so that the amplification product (AP) contains essentially only amplified sequences that comprise a specific target, suitable for detection by hybridization.
机译:从包含许多彼此分离的材料片段(B')的遗传材料(B)中扩增遗传信息是新的,包括使用PCR和引物(P)进行互补,这些引物与(B)中许多位置的结合位点互补。通过使用PCR引物(P)来扩增遗传信息(B)的遗传信息,该遗传材料包含许多彼此分离的材料片段(B'),该引物与(B)中许多位置的结合位点互补。每个结合位点与预定长度的靶序列的一个(B')相邻,使得扩增产物(AP)基本上仅包含包含特定靶的扩增序列,该序列适合通过杂交检测。

著录项

  • 公开/公告号DE10242359A1

    专利类型

  • 公开/公告日2004-03-25

    原文格式PDF

  • 申请/专利权人 ALOPEX GMBH;

    申请/专利号DE2002142359

  • 发明设计人 MANN WOLFGANG;HOLINSKI-FEDER ELKE;

    申请日2002-09-12

  • 分类号C12Q1/68;C12N15/63;

  • 国家 DE

  • 入库时间 2022-08-21 22:43:57

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