For the analysis of single nucleotide polymorphisms, in human genome analysis, melting point curves are constructed to show the difference between wild type and mutated DNA probes

摘要

For the analysis of single nucleotide polymorphisms (SNP), with DNA micro-arrays and total internal reflectance fluorescence (TIRF), nucleic acids are developed at short DNA probes in a micro-array at different temperatures. Melting point curves are constructed from the measured values, and the difference is generated between the probe for wild type DNA and the probe for the mutated DNA. The position of the curves gives an unequivocal decision whether it is a homo zygote or hetero zygote DNA for the SNP, and what type of homo zygote is present. A semiconductor laser diode (1) is focused (3) through a lens (2) to the coupling edge of a TIRF chip (4), with bio-molecules (5,6) on its surface. The molecules carry a luminescent marking, and their luminescence is detected.