Preparing endothelial cells with angiogenic or non-angiogenic phenotype, useful in screening for antiangiogenic, e.g. cytostatic, agents, by growing cells on specific media

摘要

Preparing endothelial cells (EC) with either a non-angiogenic phenotype (EC1) or an angiogenic phenotype (EC2), is new. Preparing endothelial cells (EC) with either a non-angiogenic phenotype (EC1) or an angiogenic phenotype (EC2) comprises: (a) incubating EC, particularly from the aorta, in medium that (a) contains estradiol and a growth factor (especially vascular endothelial growth factor, VEGF) for EC2 or (b) lacks these supplements, for EC1; (b) individual clones are removed using a micropipette and grown until confluent, as confirmed by phase-contrast microscopy; and (c) EC1 and EC2 are selected by verifying the phenotype of cells from (2), using at least one in vitro proliferation, migration and/or angiogenesis test. An Independent claim is also included for EC1 and EC2 produced by the new method.