New DNA encoding mutant form of LysE protein, useful for transformation of methanol-utilizing bacteria for production of lysine and arginine, also new transformants

摘要

DNA (I) that encodes a mutant (II) of the LysE (lysine export) protein of a coryneform bacterium, or its homolog, is new. DNA (I) that encodes a mutant (II) of the LysE (lysine export) protein of a coryneform bacterium, or its homolog, is new. (II) is a 236 amino acid (aa) sequence (2), reproduced, in which at least Gly56 has been replaced by a different aa, optionally with one or more other aa substituted, deleted, inserted or added. When (I) is introduced into a methanol-utilizing bacterium it confers resistance to a lysine analog (III). Independent claims are also included for: (1) bacterium (A) of the genera Methylophilus or Methylobacillus into which (I) has been introduced, in expressible form, and which can produce L-Lys or L-Arg; and (2) producing L-Lys and L-Arg by culturing (A).