Characterization of single-stranded nucleic acid by melting analysis of the secondary structure using a specific dye - a double-stranded nucleic acid

摘要

A new method of characterizing the nucleic acid. Double-stranded nucleic acid, nucleic acid - in combination with a specific dye, while the double-stranded structure of one or more nucleic acids within the dye, to form a detectable complex. Then exposed to varying temperature combination, by measuring the fluorescence of the dye, to determine the melting temperature of the double-stranded structure. Then, in some embodiments, as compared to the melting temperature profile is generated every other nucleic acids, the melting temperature profiles, to identify differences between nucleic acids were compared.