Quantitatively accurate and efficient DNA of sensitivity by real-time PCR

摘要

The sensitivity of regions of genomic DNA from chromatin to DNA-modifying agents are quantitated using real time PCR with careful use of reference regions and standard curves for improved accuracy over previous methods. A wide variety of DNA modifying agents are used to modify DNA, including single strand breakers, which allows analysis of a wider variety of DNA structures with greater precision than was previously possible. Methods also are provided that allow fine detailed analysis of hypersensitive DNA regions from as little as 100 cells. These methods also potentiate the investigation of more fine details of chromatin fiber folding and nucleosome-nucleosome contact.